The poster has the following instructions for the treatment of snake bite:- "A ligature, that is, a strong string or narrow strip of clothing if no string is to be had, should be at once applied near the bitten part, and between the bite and the heart. tight enough to stop the circulation. The bitten part should then be immediately cut out by making, in the manner shown in the engravings, two curved incisions, which should be, where practicable, a quarter of an inch deep. The wound should then be well sucked for then minutes, by persons who have no wound, cut, sore or ulcer in their mouths, or on their lips. When the bite is on the head, face or body, or on any place where a ligature cannot be applied, the bitten part should be kept tightly pinched up till it can be cut out." The poster was authorised by T.R. Wilson, Secretary of Melbourne's Central Board of Health, between 1865 and 1881. It was printed by John Ferres, Government Printer, Melbourne, 1865-1881. The Central Board of Health, Melbourne, was in existence between 1855 and 1889. This poster has significance as an early record of public health instruction issued by the Central Board of Health in Melbourne for the treatment of snake-bite. The materials used to mount and frame the poster are also of significance, with the printing being done on fabric, and the newspaper inserted between the poster and the backboards.Timber-framed rectangular poster with printed instructions for treating a snake bite. The poster is printed on fabric. Between the poster and the backboards is a layer of newspaper, revealed by the damaged fabric. The back of the frame is filled by three vertical timber panels that are strengthened by three horizontal timber slats across the top, centre and bottom. The timbers are fixed in place with nails. The edges of the frame at the back have remnants of paper. Two metal eyelets are inserted into the top edge of the frame. The poster was issued by the Central Board of Health in Melbourne and printed by the Government Printer in Melbourne. It has the British Coat of Arms on top, diagrams and text, plus sections of newsprint.Symbol of [British Coat of Arms] between the letters "V." and "R." Headline "CENTRAL BOARD OF HEALTH" Subheading "WHAT TO DO IN CASE OF SNAKE-BITE" Instructions are printed on the poster. Diagrams include a bite on the knee and bites on the hand. Publisher "Central Board of Health, Melbourne, 28th February ----" "(By Order of the Board), T.R. WILSON, Secretary" "BY AUTHORITY, - - - - - - - - -, GOVERNMENT PRINTER, MELBOURNE" Newsprint includes:- "Duties in the bay were put ... --rried out. On ... harbour boat's cr-- ... , and formed of sufficient ... given ... to vessels in distress, and ... life-b- ... the help of a few..." and "last, a Gold English ... engraved -- cove-- to leave it at F.P. ..."flagstaff hill, warrnambool, maritime village, maritime museum, shipwreck coast, great ocean road, central board of health, melbourne, t.r. wilson, secretasry, government printer, john ferres, snake-bite, treatment, first aid, 19th century, poster, government health announcement

Augustus Mueller was a medical practitioner in Yackandandah and District who was convinced of the efficacy of strychnine as an antidote for snakebite. He believed that small doses of strychnine would stimulate the nervous system while being insufficient to cause the death of the patient by poisoning. The patent for his antidote was granted on May 10th 1891. His treatment gained some acceptance in the general community but was widely condemned in the medical profession. Dr Mueller investigated treatments for typhoid following a local outbreak in Beechworth, Victoria. He also received several prizes for his work as a vigneron. Dr Mueller died in Yackandandah, Victoria on 31st December 1898. The instructions included the following information:- "Inject now only half a syringe full, or, if the patient objects to the needle, let him take ten drops of the antidote in a little cold water every half-hour, until he or she feels a little stiffness about the jaws, slight pain in the back of the neck, and jerking of arms and legs on moving them. These are the effects of the antidote. They are quite harmless, and must be produced to ensure the safety of the patient".This item is significant because it was A leather case embossed with gold embossed writing. It contains items as part of a snake bite kit and detailed instructions for its use.On lid DR. MUELLER'S ANTIDOTE FOR SNAKE BITE WITH HYPODERMIC SYRINGE & DIRECTIONS FOR USE. PREPARED BY M. S. ROME YACKANDANDAH.snake antidotes, doctor augustus mueller, northeast victoria identities

Prior to the first antivenom development in Australia, many of the snake men had a vast array of snakebite remedies they either used for themselves in the case of bites or pedaled to the public. Treatments such as ammonia, strychnine, chlorinated lime, potassium permanganate, suction caps, alcohol, gunpowder, petrol, toad urine, iodide swabs and pig face plant juice were some that were used and sold. The first antivenom produced in Australia was in 1930 for tiger snake bites. Subsequently, in response to public pressure, other antivenoms were produced. Taipan, 1955; Brown snake, 1956; Death Adder, 1958; Papuan black snake, 1959; Sea snake, 1961; and the polyvalent, 1962.Black box with hinged opening and gold leaf printed text on the top. Inside the box is lined with blue satin and velvet, and contains one (1) syringe with glass chamber and metal plunger and black rubber stopper, one (1) glass bottle with a label stating it contains chloride of lime, one (1) glass bottle with a label stating it contains pure alcohol and two (2) needles with metal connectors, one of which is broken. There is also a small wooden block with a groove in the top of it.Gold lettering on top of box: CHLORIDE OF LIME ANTIDOTE / FOR / SNAKE BITE / FELTON GRIMWADE & CO. / MELBOURNE.hydrochloride lime, alcohol, antidote, venom, snake bite

An account of a snakebite which occured during a gardening activity in Blackburn. The victim Mrs Mann had received enough venom from the bite to kill a child. The snake was identifies as either a copperhead or black snake. The article contained information on how to treat snake bite and advice on keeping gardens snake free.A snakebite which occured during a gardening activity in Blackburn. csl, melbourne geo, snakes, box hill hospital, mann gwen, goulopoulos, tom, black snake, copperhead snake, st vincents intensive care unit

The Gunning family owned 20 acres of orchard and farm land in High Street Lower Templestowe and kept one of these kits in each vehicle and tractor. They didn't experience a snake problem until the late 1950's when land sub division around their property forced snakes into the area. The family lost one dog to snake bite in the early 1960's.Consists of a Wallet, tourniquet and lancet . 1.Wallet - small red plastic wallet with one press stud to close. Printed in gold on front: 'Sanax Snakebite Kit Sanax Pty Ltd Melbourne Australia 2.Tourniquet - rubber tourniquet looped through a metal clip used to tighten tourniquet. ' Sanax ' embossed on flat of metal clip. 3.Lancet - a three chambered cylindrical container made of red plastic. One end removes to reveal a lancet/ blade, the other end acts as a handle for the blade. Container holds instructions on a small roll of paper. The centre of the container can be be unscrewed to access crystals of permanganate of potash to put on the woundmedicine, first aid

Liquor ammonie was an antidote for snake bites in the late 19th century. George Halford, a medical professor at Melbourne University promoted ammonia injections to stimulate the heart and reinvigorate the blood. Inspired by experiments in dogs, his suggestion to intravenously dose snakebitten patients with an acknowledged poison was certainly controversial. The prevailing treatments, for doctors and laity, were ligature, cutting and sucking the wound, rest (or vigorous exercise), and prodigious quantities of alcohol. Support for ‘Halford’s injection’ nevertheless grew: Victorian doctors, chemists and citizens extolled its effectiveness in both human and animal patients. (Info. from Spectacular serpents: Snakebite in colonial Australia)This item is a useful research tool.A one sheet tabular list of cases of snake bite treated by the injection of liquor ammonie. There are pinholes in each corner.on front - Harry Richardsonsnake-bite australian-medical-journal halford-george

Small wooden canister with a screw in blade at one end and a screw on cap at the other end which held condys crystals.

At the time of manufacture, circa 1940's, the first aid treatment for snake bites, was to lance(cut) open the bite, wash it out and apply a ligature or tourniquet between the bite and the heart. This method was abandoned later after scientific evidence suggested that it was better to stop the blood system from carrying the poison to the heart (pressure immobilization). Australian snakes do not have long fangs therefore deep incisions into main arteries is rare. This research took many years to develop therefore the information supplied with the "Sanax" kits of this era is well outdated. Rural areas were more attuned to the possibilities of snake activity on farms, in sheds and in out houses and farm homesteads. This was not the case for mainly city and large townships environments. The mice plagues in wheat areas during the warmer month s has always been a high risk time for encountering snakesThese snake bite kits were issued to workers on the Kiewa Hydro Electricity Scheme. Some of these snake bite kits were kept at doctor's surgeries e.g. Bogong Village, House 13. The kit was carried by bushwalkers, scouts, farmers and high plains cattlemen. This item is of significance because it demonstrates the main dangers faced by all rural industries and towns, especially remote areas, and the portable remedies that where required as a necessity of the Australian bush environment. This item was in use at a time when the air ambulance and rescue facilities were not in existence and remote locations were more isolated and the availability of anti venom was hard to obtain locally.This item is a red coloured cardboard box(250 - 300 g/m) with white writing and containing the following 12 items. 7 clear red coloured capsules, 2 opaque red plastic capsules, 2 wooden capsules and 1 brass screw on capsule. The capsules are divided into two sections (compartments) horizontally. Each compartment has a screw lid. One compartment contains a lancet (for piercing the snake bite) and the other compartment contains the "Condy's" crystals compete with tightly rolled instructions.On top of the pull up lid, and flanked by two coiled snakes (at the strike position) is printed: "Trade Sanax Mark" below this " SNAKE and SPIDER BITE OUTFIT" and below this with instructions for use". Front side: "Sanax snake and spider bite outfit" underneath "Nepean Highway Morabbin" The later printing has been severely scratched but decipherable. The back side of the box has "Note to the retailer" and due to wear no more of the writing can be read. Each side of the box is printed "SANAX" snake, spider, first aid, kiewa hydro scheme, portable medical supplies

Doctor's sealed glass vial containing catgut thread and surgical needle suspended in a sterile liquid. Thread is wrapped around a card labelled "Sterilised Surgical Ligature Thread No 0"Surgical Catgut Licence No 43 Batch No April 1939medicine, snake bite, kit

One printed tea towel with first aid instructions for bleeding, burns, snake bite, asthma, heart attack, blood nose,convulsions, sporting injuries.First Aid,St John

Used to protect ankles from sticks in thick scrub as well as from snake bitesCanvas protective gaiters with leather straps (ex-army)forests commission victoria (fcv), protective clothing, bushfire

This bottle contained Condy's crystals. Condy's Crystals (potassium permanganate) was a popular domestic item for decades, its crystals usually being dissolved in water to make a purple solution for antiseptic and disinfectant purposes. It was also used for snake bite, with standard first aid texts advising that a snake bite should be cut with a knife or razor, the venom sucked out and Condy's Crystals applied to the site. (ref. Powerhouse Museum) A small rectangular four-sided brown bottle. The neck has a screw thread.On sides - POISON NOT TO BE TAKEN On bottom - F1090 Mmedical health bottle condy's-crystals

This style of first aid kit was marketed to local sports clubs, and is socially and historically significant.First Aid Kit, dark brown heavy cardboard case with metal corner and edge reinforcing and metal clips. Including collapsible wooden splint, boracic lint, gauze, linen sling, castor oil,sal volatile, anteseptic, band-aids, adhesive tape and snake bite instructions.first, aid, kit

Notes, some taken from the Bendigo Advertiser 10/71895, 12/7/1895, and 23/12/1885. Items mentioned are: Snakes of the Whipstick, Science Society, Elliott's Reef Company, Sandhurst Museum, Snakes common to Bendigo, Snake Bite, The Whipstick Water Supply - Bendigo Advertiser 23/12/1885, Are Slides guides to Mining, Mr Wilson Froggatt's return from New Guinea, Death and a short obituary of Dr MacGillivray, Some of the work of watercolour and pencil artist, Mr R H Nancarrow, of Neilborough, and a description of Dr Mac's new residence on the lower corner of Rowan and Forest Streets.bendigo, history, early bendigo, peter ellis collection, bendigo advertiser 10/7/95 pg 3 & 12/7/95, snakes of the whipstick, school of mines, r brough smyth, science society, dr macgillivray, eaglehawk prospecting committee, elliott's reef company, sandhurst museum, prof halford, mr r bloomfield rees, professor mccoy, mr vahland, mr robshaw, the whipstick water supply, bendigo advertiser 23/12/1885, mr nancarrow, mr wilson froggatt, sir edmund strickland, geographical society, paul howard, isabella shields, sandhurst mining board, mr r h nancarrow - artist - watercolour & pencil, arnott's patent ventilators

This photograph shows a young Wilfred Busse wearing work clothes in the Australian bush. These clothes were all chosen for their practicality in the Australian bush, and create together a distinctive style that is easily recognisable. While it initially appears that he wears long boots, he actually wears shorter shoes with an additional leg protection called gaiters. These protected the legs and were particularly useful to Australian settlers to protect from snake bites. His trousers, or breeches, are of a similar style to the ones issued as Australian Army regulation during the First World War, which were baggy around the thighs and became tighter below the knee. His long shirt protects him from the harsh Australian sun, and he wears an iconic Akubra hat for sun protection on his face. It is assumed that this photograph shows Busse when he was working on a Station in Victoria in his twenties, which is supported by the style of his clothing and his age.This photograph is significant as it shows Wilfred Busse as he appeared in his younger years, presumably on a Station in Victoria. It also provides a primary resource of working clothes for white settlers in the early 20th centuryBlack and white rectangualr photograph on matte photographic paper in wooden frameaustralian settlement, settlement, workmen, work, clothing, workwear, bush, wilfred busse, busse, busse author, chiltern, chiltern athenaeum, chiltern athenaeum museum, 1920s

Living, working, recreational activities in remote areas require precautions including a First Aid Kit.Brown rectangular case with a brown curved handle at one end and 2 clip fasteners at the front with a sliding lock fastener in the middle of them. All corners are reinforced. The inside has a lift out box, bandages, medication, tourniquet, snake/spider bite medication. There is a First Aid / Emergency / Instructions booklet inside the top of the case's lid.Outside on the lid: "Sanax" / First Aid / Casefirst aid kit, first aid case, sanax, remote areas

Supplied to S.E.C. workers. This equipment was distributed to State Electricity workers working on the Kiewa Hydro Electric Scheme during the 1940's and 1950's.Historical: S.E.C. workers worked in remote areas where they didn't have easy access to medical facilities. Shows the development of snake bite treatment and spider bites.Cardboard brown box without lid for holding two wooden cylinders. Each cylinder can be pulled apart showing one end with a lancet which is used to cut the skin after a snake or spider bite. Each wooden container has instructions in very small red writing. Also in the box are instructions for Snakebite and Spider Bite. This leaflet is written in red on white paper and has more detail than the instructions on the lancets. It is made up of a front and back page opening up to the middle page.Pamphlet: 'The / Treatment / of / Snakebite / and / Spider Bite' and at the bottom 'Sanax'first aid, snakebite, spider bite, sanax company, state electricity commission of victoria, workers, medical

This small, portable 1930s Sanax First Aid Case has been strongly constructed, with corners reinforced with metal to take knocks and bumps, so it could be quickly transported to the site of an emergency. Having these supplies organised into a kit made them easily accessible and reduces time to take them to the site of the accident. It was possibly designed for use in factories because the booklet in the case states that the kit complies with “Part 1, Victorian Factories Regulations”. The text of the printed brand “Sanax First Aid Case” is right-way up when the case stands vertically on its hinged side. In modern times people are well aware of the importance of quick treatment when accident and injury occur. However, before the first commercial First Aid Kit was made by Johnson & Johnson in 1888, people had little knowledge about treating injuries and lacked information about suitable supplies to keep on hand for emergencies. They were often unaware of how to help in that critical time before the doctor or other assistance arrived, a particularly important time for the many people living in remote areas. A quote from Johnson’s & Johnson’s 1888 price list explains “It is a fact, which is everywhere being recognized, that many lives are lost and much suffering entailed in such accidents on account of the lack of the simple but necessary articles required to afford prompt assistance to the wounded.” One example of the value of First Aid assistance to community groups is shown in an article from the Weekly Times, 29th November 1930. It records a report from the Annuello Branch of the Younger Set (a Country Women’s Organisation), telling that on Armistice Day their president Mrs Jamieson, presented the Annuello School with the gift of a Sanax Red Cross First Aid outfit, which was accepted as being “of great practical use to the scholars.” (Annuello is a remote wheat growing area in the Mallee region of North Western Victoria, which became a soldier settlement area after World War I. There is a strain of wheat named ‘Annuello’ due to its suitability for that area. ) The Sanax Case in our Collection contains instructions, equipment and medical items suitable for use in emergency situations. The Case was one of 42 patterns available from Sanax that conformed to ‘Part 1, Victorian Factories Regulations’. It includes items made by Sanax Company and by Burroughs Wellcome & Co. (Australia) Ltd., Sydney, NSW. A quote at the back of the First Aid Emergency Instructions booklet says: “Sanax products are made in Australia by or under the supervision of qualified chemists, from the highest quality materials. They are dependable for the purposes written on labels.” BOOKLET included in First Aid Case: “SANAX” First-Aid Emergency Instructions has orange cover and white pages, joined in the centre by two staples. Booklet contains First Aid Instructions for general events listed in alphabetical order. It also contains an indexed sections headed “Poisoning, and what to do” written by S.A. Burrows, Ph.C., Vuc and N.Z. There are instructions and diagrams on how to perform Artificial Respiration. There are advertisement for Sanax products throughout the booklet that include; - Sanax Ambulance Stretcher for timber mills, mines, ships and quarries - Saw dust masks (porous rubber) for workers in dust, paint or duco sprayers Inside cover lists Sanax’s Australian made products including - tablets and powders for headaches, neuralgia, influenza, colds - snuff for Catarrh that is “quite harmless” - First Aid Cases that come in a range of 42 patterns - sunburn preventatives and treatments - healing salve for carbuncles, pock, pimples, boils, varicose ulcers etc. - snake bite outfits and kits LEAFLETS included in First Aid Case: (1) Tannafax Tannic Acid Jelly. Tannafax should be kept at hand in every home. It should be applied direct from the tube and used with neither oil nor grease. Where a large area has to be covered the clamped end may be torn or cut off to give a wider mouth to the tube. Collapsible tubes of different sizes. Made in Australia. Burroughs Wellcome & Co. (Australia) Ltd. (Incorporated in England). Sydney, NSW. Assorted Houses, London, New York, Montreal, Cape Town, Milan, Bombay, Shanghai, Buenos Aires. Copyright A. 1817, J. 9463 (2) Tabloid. The strong thing is the just - - . Tabloid marks the wor - - Burroughs Wellcome & Comp. The use of the word is to enab – the prescriber, dispenser and patient to get the right thing with one short word, instead of the firm’s long name. If another maker apply the word to his product, the act is unlawful. Tabloid is our trade mark and brand. If a vendor disregard it in dispensing or selling, the act is unlawful for the same reason. We prosecute both offenders rigorously, in the interest of prescribers, dispensers, patients and the owners of the trade mark. Please inform us of any instance of either offence. Burroughs Wellcome & Co. (Australia) Ltd. (Incorporated in England). Telephone Number - M 4184 (4 lines) All communications to G.P.O. Box No. 1185 DD. Copyright Sy. 20. & J 9894. Medicines and Equipment included in First Aid Case: - Absorbent Cotton, Sanax, for absorbing blood or drying a wound. As a swab for washing wounds; to place above a compress to keep the heat in: or as a pad to protect wounds or fractures. The Sanax Co. Manuf. Chemists, Melbourne. Regd. Office: 5 Brunswick St, Fitzroy. N.6. - ACHE tablets, Sanax, for all aches, pains, fevers etc. Dose: 2 to 3 tablets with a draught of water, every 3 hours. Children in proportion. For influenza or colds, take the bedtime dose with a hot lemon drink or toddy. Recommended for Headaches, Colds, Influenza, Fevers, Neuralgia, Rheumatism, Nerve Pains, Sleeplessness, and Seasickness. Three Sanax Ache tablets equals one Sanax Ache powder. Each tablet contains 1.75grs. each Phenacotinum and Acety acSzilcyl, and .75grs Ammon Brom. Etc.. Sanax brand specialties are prepared by highly qualified pharmaceutical chemists and may be accepted as safe and effective for the purpose indicated on the label. The Sanax Co. Melbourne - Eye lotion, Sanax, “in eye bath full strength or diluted with equal parts of water. Sanax Co. Brunswich St, Fitzroy, Melbourne. - Iodine, Sanax, POISON, with instructions for what to do if swallowed. - Kuraburn, Sanax, Applied to the burn and allowed to dry, the pain and heat instantly disappear, and blistering is prevented. If necessary, apply again in an hours. To safeguard against burning when sunbathing, apply before exposure to the sun. If already sunburnet, use Kuraburn as directions above. Safe and harmless. Sole makers, The Sanax Co. Brunswick St. - - Vic. - Sal Volatile, Sanax, - - stimulant for - - nervous aches - - or as smelling salts Dose - - - - Solution of A- - - 5%, . The Sanax Co. Brunswick St, Melbourne. - Tannafax, Burroughs Wellcome & Co. Australia Ltd. Sydney, N.S.W., 20gm. Approx., Tannic Acid Jelly, (Tannic Acid with 0.5% Phenol in a water-soluble base) for burns and scalds. A.N. 15050, p188, logo of a unicorn. Apply lightly, allow to dry, and bandage loosely. Do not apply oil or grease. - bottle wrapped in brown paper, unknown contents, paper adhered to bottle. - dish, kidney shaped, metal, white enamel with black rim - eye bath, green, plastic or Bakelite SANAX COMPANY The Sanax Company was at the address of 5 Brunswick Street, Fitzroy [Melbourne] at least as early as November 1924, as shown by its advertisement of Ache Powder in the Weekly Times, 8th November 1924. It was still at this address in September 1951, when it advertised First Aid outfits and components in the Post Master General’s section of the Commonwealth of Australia Gazette. REFERENCES: Annuello, Victoria; Wikipedia, https://en.wikipedia.org/wiki/Annuello,_Victoria Annuello Younger Set, Branch Activities and Local Reports, Country Women’s Organisations, Weekly Times, 29 November 1930, Trove http://trove.nla.gov.au/newspaper/article/224921009?searchTerm=%22sanax%22%20and%20%22melbourne%22&searchLimits=# Commonwealth of Australia Gazette, Issue 32, 24th April 1915, https://www.legislation.gov.au/file/1915GN32 [Johnson & Johnson Price List, September 1, 1888, p. 20. From our archives], Celebrating the 125th Birthday of the First Aid Kit , The Story of Johnson & Johnson, , http://www.kilmerhouse.com/2013/06/from-1888-to-2013-celebrating-the-125th-birthday-of-the-first-aid-kit/ Post Master General’s section of the Commonwealth of Australia Gazette, Issue No. 73, Thursday 27th September 1951 http://trove.nla.gov.au/newspaper/article/232185299?searchTerm=%22sanax%22%20and%20%22fitzroy%22&searchLimits= Sanax First Aid Emergency Instructions, by S.A. Burrows, publisher Sanax Ltd. Fitzroy, Victoria, 1930-1939 English, book, Illustrated edition, Trove http://trove.nla.gov.au/version/40948895 Access to emergency medical help in early settlement days of Victoria could take quite some time, especially in remote areas. From 1888 First Aid Kits and instructions became available for work sites, offices, community groups and individuals, helping to bridge the gap between the accident and the arrival of medical assistance. This portable Sanax First Aid Case is an example of portable medical equipment made in Melbourne, Australia, in the 1930’s and available to the public. It contains a range of items plus information to be used in a variety of injuries and emergencies in in factories, households, businesses and local communities, and instructions on their use. First Aid Case, portable, Sanax First Aid Case. First Aid kit in strong black cardboard carry case with metal reinforced corners, metal hinges on lid, metal catch and leather carry handle. Inside lid is a vertical strap with narrow gap behind it. Base is divided into two compartments. Manufactured by Sanax, Fitzroy, Melbourne, C. 1930-1939 Contents include "Sanax" First Aid instructions booklet, 2 leaflets, metal kidney dish enamelled in white with black trim on edge, green plastic or Bakelite eye bath, eye lotion, Tannafax tannic acid jelly, Sal Volitile, Kuraburn, Iodine, Argyrol, ACHE tablets, absorbent cotton in cardboard box, gauze bandage, and UNKNOWN wrapped bottle. Printed in gold on lid of case “SANAX” FIRST AID CASE. Most of the contents, as well as the case, show the “SANAX” brand. Some contents are inscribed Burroughs Wellcome & Co. (Australia) Ltd., flagstaff hill, warrnambool, shipwrecked coast, flagstaff hill maritime museum, maritime museum, shipwreck coast, flagstaff hill maritime village, great ocean road, first aid items, first aid kit, emergency first aid, medical emergency kit, home emergency kit, industrial emergency kit, sanax company fitzroy melbourne, burroughs wellcome & co. (australia) ltd, thos. urquhart & son pty. ltd. melbourne, sanax first aid case, sanax first-aid emergency instructions, part 1 victorian factories regulations, tabloid medical supplies